Engineered hybrid scaffolds of poly(vinyl alcohol)/bioactive glass for potential bone engineering applications: Synthesis, characterization, cytocompatibility, and degradation

Abstract

The synthesis, characterization, preliminary cytocompatibility, and degradation behavior of the hybrids based on 70 Poly(vinyl alcohol) and 30 bioactive glass (58SiO 2-33CaO-9P 2O 5, BaG) with macroporous tridimensional structure is reported for the first time. The effect of glutaraldehyde covalent crosslinker in the organic-inorganic nanostructures produced and, as a consequence, tailoring the hybrids properties was investigated. The PVA/BaG hybrids scaffolds are characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray Microcomputed tomography analysis (μCT). Cytotoxicity assessment is performed by the MTT method with VERO cell culture. Additionally, the hybrid in vitro degradation assay is conducted by measuring the mass loss by soaking in deionized water at 37°C for up to 21 days. The results have clearly shown that it is possible to modify the PVA/BaG hybrids properties and degradation behavior by engineering the structure using different concentrations of the chemical crosslinker. Moreover, these hybrid crosslinked nanostructures have presented 3D hierarchical pore size architecture varying within 10-450μm and a suitable cytocompatibility for potential use in bone tissue engineering applications. Copyright © 2012 Hermes S. Costa et al.