1H NMR of 5′CGCGTATATACGCG3′, a duplex and a four-membered loop

Abstract

The deoxyribonucleotide 5′CGCGTATATACGCG3′ was synthesized and studied by NMR methods. This short, fully palindromic duplex also forms a hairpin under certain conditions described within. As such, it is considered to be a model for cruciform formation. We show that this sequence forms a four-membered loop and a duplex in solution. The duplex is shown to be a normal, B-DNA like helix, while the hairpin is shown to be a four-membered AT AT loop. © 1991 Oxford University Press.