Biological and molecular characterization of Prunus necrotic ringspot virus isolates from three rose cultivars

Abstract

Prunusnecrotic ringspot virus (PNRSV) is a rose and stone fruit tree pathogen. Three different PNRSV isolates, originating from three rose cultivars were studied. These PNRSV isolates were characterized using molecular techniques. Nearly the complete nucleotide sequence (1,630 nucleotides) of RNA3 of the isolate PNRSV-R1 has been determined (GenBank Acc. No. DQ003584). The sequence of the MP gene of the PNRSV-R1 isolate was determined, the first such results for a rose-derived PNRSV isolate. The reaction of PNRSV infection on test plants was also investigated. Cucumis sativus cv. Wisconsin, Cucurbita maxima cv. Buttercup and Cucurbita pepo cv. Melonowa Żółta appeared to be the most useful test plants for the differentiation of isolate-specific pathogenicity. © 2011 The Author(s).