Genetic variation in polymorphic males of Callosobruchus maculatus (Coleoptera: Bruchidae) by RAPD-PCR

Abstract

Callosobruchus maculatus (Coleoptera: Bruchidae) is a stored grain pest of Vigna radiata (L) (Mung) and shows polymorphisms in males as well as females. These polymorphs are differentiated on the basis of colour pattern of elytron and pygidium. The genomic DNA of all these polymorphic forms has been analysed using RAPD-PCR technique with 2 random decanucleotide primers. Primer-12 (5′-AAGAGCCCGT-3′) and primer 17 (5′-TGCCGGCTTG-3′) which amplified variable number of distinct bands revealing bands unique to each form and common bands among them depicting the genetic variations as well as similarities at molecular level and a diagnostic band common to all abnormal forms is also obtained. © 2006 The Japan Mendel Society.