Effectiveness of Modified Sabouraud Medium in Relation to the Bacterial Growth

Abstract

Among the diseases caused by fungi, candidiasis and aspergillosis are the most frequent. In the bacteriological routine, there are different culture media used for cultivation of bacteria and fungi. Sabouraud dextrose agar is widely used for the clinical samples cultivation. In some cases, the biological samples show a mixed population of bacteria and fungi. Three culture media for the study were prepared: Mycosel, commercially available fungal medium (Sabouraud dextrose agar), and the Sabouraud dextrose modified medium (study media) with different dilutions of an antibiotic. The effectiveness of the study medium was observed from the comparison of the results of the cultures after the cultured of different samples of selected bacteria. The Modified Sabouraud medium showed effectiveness of 100%. The modified medium proved to be fully efficient and effective in both controlled tests because they completely inhibited the bacterial growth, favoring the acquisition of fungus pure cultures. We can conclude that the Sabouraud medium with the modification proposed in this study proved to be more effective in relation to the Mycosel or Sabouraud media.