The effect of cytoglobin gene inhibition on fibroblast keloid cells proliferation

Abstract

Cytoglobin (Cygb) is a new protein from the globin family whose function is reported to be either a tumor suppressor gene or oncogene. The aim of this study was to determine the function of Cygb on fibroblast keloid cells proliferation. The relative expressions of Cygb were compared between the siRNA (+) Cygb and siRNA (-) groups using qRT PCR, and their effect on cells proliferation, which was determined using the MTS assay. The results showed that the expression level of Cygb on siRNA (+) Cygb group were decreased compared to siRNA (-) group (0.315 vs 1.056; p = 0.000). However at the level of cell proliferation, there were no significant differences between the two groups (1.489 vs 1.359; p = 0.087). Based on the results, it is concluded that Cygb has no effect on fibroblast keloid cells proliferation.