Creating new opportunities in process control through radio frequency impedance spectroscopy

Abstract

[...] dielectric spectroscopy only measures viable cells and is immune to both lysed cells and other debris (e.g. carriers) in the suspension. Bottom: estimated average cell diameter from online capacitance (solid line) and ViCell system (crosses). [figure omitted; refer to PDF] Because RF spectroscopy allows differentiation between biomass and cell size, additional analysis of the cell culture growth curve can be used to determine optimum parameters.