Validation of UV-VIS spectrophotometric method for the determination of Calcium in Maman (cleome gynandra l)

Abstract

One of health problems in Indonesia is stunting. Energy and nutrients intake are important factor in the occurrence of stunting conditions. The intake of micronutrients to prevent stunting are vitamin A, zinc, iron, iodine, and several other micronutrients such as calcium and phosphorus are also important in children's linear growth. One of the plants which is a natural resource owned by Provinsi Riau and can be used as an alternative food to obtain calcium is Maman (Cleome gynandra L.). The analytical method used ultraviolet-visible spectrophotometry based on the complex formation between murexide and calcium. The optimum wavelength obtained is 530 nm with a r value = 0.9989. The LOD value obtained was 0.013 ppm and the calcium content in maman leaves was 172.64 ± 4.91 mg/g.