Voltammetric Behavior Of Trimetazidine Hydrochloride At Pre-Anodized Carbon Paste Electrode And Its Determination In Pharmaceutical Formulations

Abstract

A functional pre-anodized carbon paste electrode (PACPE) was prepared by 35 successive cycles of scans between 1.0V-1.8V in 0.2 mol L-1 NaOH solution. The electrode reaction mechanism of trimetazidine hydrochloride (TMZ) was carefully studied by CV method and was determined as a two-electron/two-proton process, which is put forward for the first time. Experiment showed that the electrostatic attraction between the protonated TMZ and the negatively charged PACPE surface, both were affected by pH, was the dominant factor influencing the adsorption and the peak current. The PACPE exhibited excellent electrocatalytic effects towards the oxidation of TMZ. In the optimum conditions, the oxidation peak current was linear with TMZ concentration in the range of 5.0×10-7—5.0×10-5 M (r=0.998), and the detection limit was estimated to be about 1.5×10-7 M. This method can be successfully applied to the determination of TMZ in pharmaceutical formulation.