Circular RNA hsa-circ-0007142 Is Upregulated and Targets miR-103a-2-5p in Colorectal Cancer

Abstract

Circular RNAs (circRNAs) are a large class of endogenous noncoding RNAs that regulate gene expression and mainly function as microRNA sponges. This study aimed to explore the aberrant expression of circRNAs in colorectal cancer (CRC). Using a circRNA microarray, we identified 892 differentially expressed circRNAs between six pairs of CRC and adjacent paracancerous tissues. Among them, hsa-circ-0007142 was significantly upregulated. Further analysis in 50 CRC clinical samples revealed that hsa-circ-0007142 upregulation was associated with poor differentiation and lymphatic metastasis of CRC. Bioinformatic analysis and luciferase reporter assay showed that hsa-circ-0007142 targeted miR-103a-2-5p in CRC cells. Moreover, the silencing of hsa-circ-0007142 by siRNAs decreased the proliferation, migration, and invasion of HT-29 and HCT-116 cells. Taken together, these findings suggest that hsa-circ-0007142 is upregulated in CRC and targets miR-103a-2-5p to promote CRC.