Catalytic mechanism of 5-chlorohydroxyhydroquinone dehydrochlorinase from the YCII superfamily of largely unknown function

Abstract

Background:TftG is a YCII superfamily dehydrochlorinase that catalyzes conversion of 5-chlorohydroxyhydroquinone to hydroxybenzoquinone. Results:The TftG crystal structure in complex with product analog 2,5-dihydroxybenzoquinone illustrated the catalytic residues and mechanism. Conclusion:A His-Asp dyad and other conserved signature residues are implicated for catalysis and substrate binding. Significance:This is the first elucidation of a YCII superfamily protein mechanism, which helps explain their obscure nature. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.