On-column UV absorption detector for open tubular capillary zone electrophoresis

Abstract

A fixed-wavelength on-column UV absorption detector has been developed for open tubular capillary electrophoresis. The detection limits are 15 pg for isoquinoline and 250 pg for lysozyme. Detector response is linear over three to four orders of magnitude for each solute tested. This approach to detector design solves many problems encountered in the use of modified commercial high-performance liquid chromatography detectors. Examples of separations obtained by capillary zone electrophoresis with UV detection are given, including separations in non-aqueous and mixed media. © 1984.