Ram-induced growth of ovarian follicles and gonadotrophin inhibition in anoestrous ewes

Abstract

Southdown ewes in mid-seasonal anoestrous were exposed to rams for 0 h (control group), 2 h, 24 h, 40 h, 3 days, 10 days or 20 days. Serial blood samples were then taken to determine LH and FSH levels. Ewes with > 24 h ram exposure were ovariectomized immediately after bleeding, and all follicles > 1 mm diameter were dissected from the ovaries and measured. LH basal concentrations and pulse frequency increased significantly within 2 h of ram introduction, but by 24 h fell, and then remained low. FSH concentrations fell within 2 h of ram introduction and remained low. Control group ewes (isolated) had no follicles > 4 mm diameter, whereas all ewes exposed to rams had large follicles, with CL or preovulatory follicles present at 40 h after ram introduction. Ram introduction was also associated with follicle recruitment (antrum formation to < 2 mm). Follicular recruitment and development to the large follicle stage therefore occurred during a period of low plasma gonadotrophin levels and suppressed LH pulsing.