Stability indicating RP-HPLC method development and validation for the estimation of octopamine in pharmaceutical dosage form

Abstract

Objective: To develop simple and cost effective Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for the estimation of Octopamine.HCl in pharmaceutical dosage form. Methods: The estimation was carried out on SUNFIRE C18 (150*4.6mm, 5μ) using mobile phase consisting of phosphate buffer of pH (2.54) and Acetonitrile (90:10). The flow rate was 1.0 ml/min column effluents were monitored at 273 nm. The proposed method has been validated as per ICH Guidelines. Results: The retention time was 2.9 minute and the proposed method was linear in the concentration range of 5 to30 μg/ml with coefficient of correlation 0.9998. The % recoveries at 99%-101% were found to be 99.1%, 98.57%and 98.23% respectively. Conclusion: All the validation parameters were within the acceptance range. The developed method can successfully be applied for the routine estimation of the amount of octopamine in bulk and pharmaceutical dosage form. The proposed method can be used to determine the drug content in various pharmaceutical dosage forms.