Studies on antiproliferative and antioxidant efficacy of caesalpinia sappan L. Heartwood

Abstract

In the present study, cytotoxic and antioxidant potentials of Caesalpinia sappan heart wood were evaluated. The heartwood of C. sappan was collected, authenticated, coarsely powdered and subjected to solvent extraction using 70 % ethanol. Preliminary phyto-chemical screening was carried out for the hydro-alcoholic extract thus obtained. Antioxidant potential of the extract was evaluated employing in vitro methods such as DPPH assay and reducing capacity. In vitro antiinflammatory studies were also performed. The data of the results of antioxidant and antiinflammatory studies were encouraging. Besides cytotoxicity efficacy of different concentrations of C. sappan heartwood extract was studied against HEp-2, HT-29, HeLa, MCF-7, T47D, HepG2 and Vero cell lines using 3-(4,5-dimethylthaizol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The dose which showed highest cytotoxic effect was subjected to further studies to understand the Apoptosis mechanism involved. The present study depicted the anticancer and antioxidant potentials of hydro-alcoholic extract of heartwood of C. sappan.