Effects of Different Plant Extracts on Post-Thaw some Spermatological Parameters of Cryopreserved Awassi Rams Spermatozoa

Abstract

Several diluting–protecting media and different methods have been used for storage of ram semen in liquid and frozen state (Salamonand Maxwel, 2000). Addition of preservatives media for increasing storage of sperm are very important for successful artificial insemination in rams. This study was conducted on evaluate the effect of 3 plant extract. Ejaculates were collected using an electro-ejaculator twice a week during there productive season. After pooling, each pooled ejaculate was split into four equal aliquots and diluted with based Tris, Citric acid, Egg yolk, glicerol extender containing plant extracts (0.02% and % 0.04 Rosemary (Rosmarinus officinalis) (Rosemary extract -oil-soluble)); Echinacea (Echinacea purpurea) (0.5 mg/mL and 1 mg/ml) and St. John’s wort (Herba Hyperici) (0.5 mg/mL and 1 mg/mL) at a final concentration of approximately 2x108 motil spermatozoa per straw. These results indicate that that the addition of Echinaceaand Rosemary as antioxidants have more positive effects for Awassi rams on spermatological parameters.