Cellulase gene recombinant Lactobacillus colonization of chicken gut and its effect on chicken growth performance

Abstract

Cellulase gene recombinant Lactobacillus was constructed in preliminary experiments and 100 one-day-old cocks were randomly divided into four groups. Group 1 was the control group in which cocks were orally drenched with 200 μL of de Man, Rogosa and Sharpe (MRS) broth; cocks in group 2 were orally drenched with 200 μL of fermentation broth containing recombinant Lactobacillus reuteri XNY-Cel15; cocks in group 3 were orally drenched with 200 μL of fermentation broth containing recombinant L. reuteri XNY-Cel73; and cocks in group 4 were orally drenched with 100 μL of broth containing XNY-Cel15 and 100 μL of broth containing XNY-Cel73. The effect of oral drenching with recombinant Lactobacillus on chicken growth and recombinant Lactobacillus colonization of the chicken gut were investigated. Results showed that recombinant L. reuteri XNY-Cel15 and XNY-Cel73 were colonized in the crop, small intestine, and cecum; the reporter gene of recombinant Lactobacillus was detected in 30-day-old chicks, which sequences corresponding with those of the insert gene; the weights of 30-day-old chicks orally drenched with the fermentation broth containing recombinant Lactobacillus were higher than those in the control group; and the weights of chicks in the C1573 group were the most significantly high (P < 0.01).