Optimization of the various conditions of extraction for the phenolic compounds from the moringa oleifera leaves

Abstract

The main objective of this particular study is in optimizing the various methods for extracting the phenolic compounds present in the Moringa Oleifera leaves by using a method called response surface methodology (RSM). In this study, a user-defined design was used to determine the effect of the time taken for extraction, the temperature during the extraction and then the ethanol concentration (percentage) on total phenolic content (TPC). The extraction process is done on three different types of Moringa Oleifera leaves. They differ in the method in which these leaves are dried (oven dried, sun-dried and ambient air-dried). The RSM method was used to attain conditions by which the extraction can be maximized i.e. Maximum extraction of TPC of the Moringa Oleifera leaves. The conditions that gives maximum extraction of phenolic compounds were the extraction time 73 min; the extraction temperature, 44°C and the percentageof methanol 97.55. The TPC that were extracted under these conditions were 12.28, 12.65 and 12.43 MG GAE/gm DW for the samples that were dried by using different methods. The difference between the three drying methods were found (p<0.05).