Spectroscopic Analysis of the Precipitate Formed by Mixing Sodium Hypochlorite and Chlorhexidine. In Vitro Study. Part II

Abstract

The aim of this study was to analyze the precipitate formed by the mixture of 5,25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) through thin layer chromatography and nuclear magnetic resonance spectroscopy (RMN-¹H, 600 MHz and RMN-¹³C to 100 MHz) 1D and 2D spectra. Thus, the following groups were established: Group A corresponds to a pure freeze-dried chlorhexidine gluconate , Group B made-up by a combination of 2ml of chlorhexidine 2% and sodium hypochlorite 5.25% and Group C was a commercial sample of PCA (4-Chloroaniline 98%). The samples of group B were rinsed with distillated water and spinned during 15 minutes at 25°C, the supernatant was eliminated by vacuum and vacuum chamber for 72 hours without heating. Finally, the solid was grinded and dried in vacuum chamber for 24 hours without heating. Thin layer chromatography, shows that sample B were composed by more than one chemical substance and Chlorexidine, the RMN-¹³C showed that the signal of the amino group characteristic of PCA appears down field (δ/146.5 ppm) in C group, meanwhile in group B appears up field (δ/129ppm), which demonstrates the absence of PCA during the process. The analysis of Group B by RMN-¹³C results also, in different signals of low intensity that correspond to similar structures to chlorhexidine and potential aromatics derivatives with similar characteristics structures to chlorhexidine.