Quantitative Mass Spectrometry-based Proteomics

Abstract

Mass spectrometry-based proteomics, the large-scale analysis of proteins by mass spectrometry, has emerged as a powerful technology over the past decade and has become an indispensable tool in many biomedical laboratories. Many strategies for differential proteomics have been developed in recent years, which involve either the incorporation of heavy stable isotopes or are based on label-free comparisons and their statistical assessment, and each of these has specific strengths and limitations. This chapter gives an overview of the current state-of-the-art in quantitative or differential proteomics and will be illustrated by several examples.