Motility and Fertility of Bull Sperm Frozen-Thawed Differently in Egg Yolk and Milk Extenders Containing Detergent

Abstract

The purpose of this study was to examine survival and fertility of frozen bull sperm processed in several extenders containing a detergent mixture, sodium, and triethanolamine lauryl sulfate. Addition of .5% (vol/vol) detergent to whole milk was completely spermicidal. When 10% egg yolk (vol/vol) was included in the glycerol or nonglycerol fractions or both fractions of milk, sperm were equally well preserved in the presence of .5% detergent, and sperm survival following thawing at 4°C was dramatically improved by the detergent. This improved result with the 4°C thaw temperature also was obtained by adding .5% detergent to an egg yolk-Tris extender. Thawing temperatures of 30 and 50°C (latter for 15 s) were generally superior to 4°C. A fertility trial with whole milk, whole milk-10% yolk, whole milk-10% yolk-.5% detergent, egg yolk-Tris, and egg yolk-Tris-.5% detergent was conducted. The 59-d percent nonreturns, respectively, were 73.4, 65.8, 65.6, 69.5, and 73.4. Whole milk or egg yolk-Tris plus detergent were equally superior to the other extenders for bull semen. © 1987, American Dairy Science Association. All rights reserved.