Purification and characterization of β-1, 3-glucanase from the secretion of Simira glaziovii colleters (rubiaceae)

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Abstract

In this study, β-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2.

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Vieira, F. A., Da Cunha, M., Klein, D. E., De Oliveira Carvalho, A., & Gomes, V. M. (2006). Purification and characterization of β-1, 3-glucanase from the secretion of Simira glaziovii colleters (rubiaceae). Brazilian Archives of Biology and Technology, 49(6), 881–888. https://doi.org/10.1590/S1516-89132006000700004

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