Cell wall pectic (1→4)-β-D-galactan marks the acceleration of cell elongation in the Arabidopsis seedling root meristem

Abstract

Here we demonstrate that the pectic rhamnogalacturonan-l-associated LM5 (1→4)-β-D-galactan epitope occurs in a restricted manner at the root surface of intact Arabidopsis seedlings. The root surface occurrence of (1→4)-β-D-galactan marks the transition zone at or near the onset of rapid cell elongation and the epitope is similarly restricted in occurrence in epidermal, cortical and endodermal cell walls. The extent of surface (1→4)-β-D-galactan occurrence is reduced in response to genetic mutations (stp-1, ctr-1) and hormone applications that reduce root cell elongation. In contrast, the application of the arabinogalactan-protein (AGP) binding β-glucosyl Yariv reagent (βGIcY) that disrupts cell elongation results in the persistence of (1→4)-β-D-galactan at the root surface and in epidermal, cortical and endodermal cell walls. This latter observation indicates that modulation of pectic (1→4)-β-D-galactan may be an event downstream of AGP function during cell expansion in the Arabidopsis seedling root.