Studies of heat shock protein response isolated from zoom land soil bacterium (Pseudomonas spp.)

Abstract

Expression of heat shock proteins (Hsps) synthesized by bacterium (Pseudomonas spp.) during stress condition (elevated temperature) is identified and studied their association in adaptation to environmental extremes. Collection, screening, isolation and identification of zoom land soil bacterium were carried out by growing bacterial cells on selective Agar Cetrimide media. Optimization of culture conditions (incubation time and temperature) for the maximum production of cell (Dry Cell Weight) also considered for the identified organism (Pseudomonas spp.) in LB media during micro-aerobic batch culture; where maximum cell growth was found 1.80 gm/L at 10 hours incubation period and 37°C incubation temperature, while rpm was 120. Subsequently, bacterial cell was grown in LB media for 6 hours at 37°C and 120 rpm into shaking incubator, then incubated for 2 hours, where maximum cell growth was observed 2.38 ± 0.06 gm/L during elevated temperature (50°C), stimulated by heat shock proteins. Then different intracellular and extracellular soluble protein extraction, purification was attempted from soil bacterium by centrifugation, sonication, repeated freezing and thawing. SDS-PAGE was performed for the separation of heat shock protein, where protein bands intensity 90, 84, 70 and 45 kDa were found according to their molecular weight. Pseudomonas spp. can produce heat shock protein during harsh condition which can help them to survive by effective adaptation mediated by universal regulatory mechanisms affecting several pathways. Hsps are expected to play a significant role to conferring tolerance in response to changes in high temperature. They are also responsible for survival of bacterial cells and increasing soil fertility which associated with virulence. So, this study will help to understand the impact of soil microorganisms to revive the soil fertility though people burn their paddy fields after harvesting in zoom areas of Bangladesh.