Validation of Functional Connectivity of Engineered Neuromuscular Junction With Recombinant Monosynaptic Pseudotyped ΔG-Rabies Virus Tracing

5Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Current preclinical models of neurodegenerative disease, such as amyotrophic lateral sclerosis (ALS), can significantly benefit from in vitro neuroengineering approaches that enable the selective study and manipulation of neurons, networks, and functional units of interest. Custom-designed compartmentalized microfluidic culture systems enable the co-culture of different relevant cell types in interconnected but fluidically isolated microenvironments. Such systems can thus be applied for ALS disease modeling, as they enable the recapitulation and study of neuromuscular junctions (NMJ) through co-culturing of motor neurons and muscle cells in separate, but interconnected compartments. These in vitro systems are particularly relevant for investigations of mechanistic aspects of the ALS pathological cascade in engineered NMJ, as progressive loss of NMJ functionality may constitute one of the hallmarks of disease related pathology at early onset, in line with the dying back hypothesis. In such models, ability to test whether motor neuron degeneration in ALS starts at the nerve terminal or at the NMJ and retrogradely progresses to the motor neuron cell body largely relies on robust methods for verification of engineered NMJ functionality. In this study, we demonstrate the functionality of engineered NMJs within a microfluidic chip with a differentially perturbable microenvironment using a designer pseudotyped ΔG-rabies virus for retrograde monosynaptic tracing.

References Powered by Scopus

Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors

21400Citations
N/AReaders
Get full text

TARDBP mutations in individuals with sporadic and familial amyotrophic lateral sclerosis

1326Citations
N/AReaders
Get full text

How common are the "common" neurologic disorders?

1252Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Human fetal membrane-mesenchymal stromal cells generate functional spinal motor neurons in vitro

7Citations
N/AReaders
Get full text

Dissection and culturing of adult lateral entorhinal cortex layer II neurons from APP/PS1 Alzheimer model mice

2Citations
N/AReaders
Get full text

Evolving alterations of structural organization and functional connectivity in feedforward neural networks after induced P301L tau mutation

0Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Bauer, U. S., Fiskum, V., Nair, R. R., van de Wijdeven, R., Kentros, C., Sandvig, I., & Sandvig, A. (2022). Validation of Functional Connectivity of Engineered Neuromuscular Junction With Recombinant Monosynaptic Pseudotyped ΔG-Rabies Virus Tracing. Frontiers in Integrative Neuroscience, 16. https://doi.org/10.3389/fnint.2022.855071

Readers over time

‘22‘23‘240481216

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 5

71%

Lecturer / Post doc 1

14%

Researcher 1

14%

Readers' Discipline

Tooltip

Business, Management and Accounting 2

40%

Nursing and Health Professions 1

20%

Neuroscience 1

20%

Agricultural and Biological Sciences 1

20%

Article Metrics

Tooltip
Social Media
Shares, Likes & Comments: 75

Save time finding and organizing research with Mendeley

Sign up for free
0