Expression of signaling molecules related to wnt pathway in cleft palate induced by retinoic acid during perinatal stage

Abstract

Signaling pathways have been shown to participate in the process of Retinoic acid (RA) - induced cleft palate (CP). In our current study, the signaling molecules of GSK-3β, Fzd3 and β-TrCP, which all relate with Wnt pathway, were screened from the palate tissues of the RA-induced CP in mice by the Gene-chip Technology. But, their expression pattern and level in palates during perinatal stages have not been known yet. In the studies, mRNA level of GSK-3β, Fzd3 and β-TrCP were detected by quantitative real-time PCR, and showed significant difference between the Embryonic day 18 (ED18) before birth and Postnatal day 0 (PD0) after birth, as well as between the RAinduced CP and wild type during the perinatal stage, respectively. And the localization pattern of GSK-3β, Fzd3 and β-TrCP proteins in palates was also characterized. Our data indicates that the Wnt signaling pathway may involve in the RA-induced cleft palate during peirnatal stage. © 2012 The Hard Tissue Biology Network Association Printed in Japan, All rights reserved.