Phytochemical Profiling by Hyphenated Technique and Invitro Enzymatic Anti-Diabetic Activity of Ethanolic Extract of (Oryza sativa. L Indica)

Abstract

Diabetes mellitus is one of the metabolic disorders caused by insufficient secretion of insulin from pancreas this leads to increase level of blood glucose level. This causes severe side effects like retinopathy, neuropathy and so on. In India the common food is rice (Oryza sativa) for their day-to-day life which is having excessive carbohydrate and less fibre content. Hence, there is a chance of increased glycaemic index level in our body. To overcome this problem there is an alternative food black rice (Oryza sativa. L Indica) contains more fibre and less carbohydrate when compared to white rice. In this research we focussed the phytochemical analysis and invitro pharmacological evaluation by using enzymatic assay method with the use of ethanolic extract of Oryza sativa. L Indica by cold maceration process. Phytochemical analysis of extract revealed that presence of various phytoconstituents like flavonoids, saponins, alkaloids, tannins, phenols and glycosides. In Aldose reductase inhibition assay extract showed significant inhibitory property with and IC50 value of 238.7μg/ml. Results of α-glucosidase inhibitory assay showed IC50 value of 383.2μg/ml and IC50 of 228.6 2μg/ml for α-amylase inhibition activity. The anti-diabetic potential may be due to the presence of flavonoids and phenols present in the extract. In these researches it was proved through invitro anti-diabetic potential using enzymatic inhibition activity. In future, through in vivo method, the same activity will be carried out for further research development towards diabetes. By concluding that black rice can be taken in daily diet by all common people without any complications.