Electron microscope analysis of partial denaturation of F factor deoxyribonucleic acid

Abstract

Partial denaturation pattern of sex factor deoxyribonucleic acid of E. coli was studied by electron microscopy. Clustering of the adenine plus thymine rich regions in one part of the molecule was revealed. The position of these regions were located on the physical map of F by analyzing the partial denaturation pattern of heteroduplexes between F and F prime factors with various parts of F sequences deleted.