A new canine cell line, named CCT, was established from the cutaneous malignant histiocytosis in a 4-year-old male Borzoi. CCT proliferated with loose adherence and doubling time was approximately 30 hr. When co-cultured with latex beads, CCT phagocytized beads vigorously. Lysozyme and vimentin were positive by immunostaining, and non-specific esterase and acid phosphatase were positive by cytochemical staining. These features indicated the cells had a histiocytic nature. Furthermore, by subcutaneous injection to nude mice CCT could successfully form tumors with the morphological and immunohistochemical features similar to the original tumor.
CITATION STYLE
Sakai, H., Nakano, H., Yamaguchi, R., Yonemaru, K., Yanai, T., & Masegi, T. (2003). Establishment of a new canine cell line (CCT) originated from a cutaneous malignant histiocytosis. Journal of Veterinary Medical Science, 65(6), 731–735. https://doi.org/10.1292/jvms.65.731
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