Chemical synthesis of the 24 RNA fragments corresponding to hop stunt viroid

Abstract

A general and practical synthetic method of oligoribonudeotides (10-20 mere) by using the cyanoethyl phosphoramidite approach was described. In this experiment 9-phenylxanthen-9-yl(Pix) and 9-(4-methoxy)phenylxanthen-9-yl (Mox) groups were employed for the 5′-hydroxyls and tetrahydropyranyl (Thp) group was used for the 2′-hydroxyl protecting groups. In addition, suitable acyl groups were introduced for the protection of the lactam functions of guanine and uracil moieties. © 1989 IRL Press.