Genetic diversity of mungbean genotypes with different resistance against mungbean yellow mosaic virus determined by micro satellite markers

Abstract

Mungbean yellow mosaic virus (MYMV) is a single-stranded DNA (ssDNA) begomovirus that is the most prevalent threats in Pakistan to mungbean crop. Ten genotypes were selected from 127 genotypes which have already been screened for resistance under field conditions. A total of 38 bands were scored, exhibited high level of polymorphism i.e., 68.42%, with amplification range of (1-7bands). Genetic analysis often primers revealed similarities in the range of 66.60-97.70%. Majority of primers were moderately informative with polymorphism information content (PIC) value (0.00-0.566) and average PIC value of all primers was 0.379. Cluster analysis inferred that genotypes showing resistant response against MYMV were present in un-clustered form in dendrogram. On the basis of phylogenetic analysis NM-2011 has higher genetic difference which reduces to the next branches showed that the susceptible (S) and moderately susceptible (MS) genotypes diverged from resistant genotypes. Diverse field response of mungbean was confirmed even on SSR markers and furthermore it is recommended that molecular markers are effective tags for genetic diversity calculation in mungbean germplasm and could be utilized for the future breeding program regarding mungbean crop.