Marine bioactive steryl esters from the red sea black coral antipathes dichotoma

Abstract

Two new marine steryl hexadecanoates, 3β-hexadecanoylcholest-5-en-7-one (1) and 3β-hexadecanoylcholest-5-en-7β-ol (2), along with seven known compounds, cholest-5-en-3β-yl-formate (3), thymidine (4), indole-3-carboxaldehyde (5), 3β-hydroxycholest-5-en-7-one (6), cholesterol (7), 22-dehydrocholestrol (8), and 24-methylenecholesterol (9), were isolated from the anthozoan black coral, Antipathes dichotoma. The structures of all isolated compounds were determined by interpretation of their spectral data 1D (1H, 13C and DEPT) and 2D (COSY, HSQC and HMBC) NMR, UV, IR, and MS. Compounds 1-5 were evaluated for their anticancer activities, employing four cancer panels; HepG2, WI 38, VERO, and MCF-7. All tested compounds, except 4, showed moderate activities. Compound 4 exhibited significant anticancer activity against VERO and MCF-7, with IC50 20.5 and 20.2μg/mL, respectively. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.