Synthesis and characterization of chitosan human like collagen/b-sodium glycerophosphate-carbodiimide hydrogel

Abstract

Injectable in situ forming biodegradable chitosan-human like collagen (CS-HLC) based hydrogels has proved to be a potential candidate as an injectable biomaterial for tissue engineering. With crosslinking agent of carbodiimide, the properties of the novel chitosan-humanlike collagen/b-sodium glycerophosphate-carbodiimide hydrogel were also examined. The gelation time, structure, equilibrium swelling and degradation in vitro and in vivo were dependent upon crosslinking and structure of composite hydrogels. The chitosan-human-like collagen/b-sodium glycerophosphate-carbodiimide hydrogel showed a desirable gelling time, swelling ratio, a smooth surface, regular porous networks and biodegradable. Therefore, the chitosan-human-like collagen/b-sodium glycerophosphate-carbodiimide hydrogels are excellent candidates for use in biomedical fields, such as in soft tissue defect filling and drug delivery.