Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1

Abstract

In this study, a sensitive and specific monoclonal antibody against VIR M1 was produced based on a new hapten. The antibody had a linear detection range of 3.77–65.51 ng/mL with an IC50 of 15.26 ng/mL and good specificity for VIR M1. Gold nanoparticle (GNP)-based lateral flow strips were assembled for rapid screening of VIR M1 in feed samples. When evaluated with the naked eye, the visual limit of detection (LOD) in feed samples was 25 μg/kg, and the cut-off value was 200 μg/kg. Therefore, this GNP-based lateral flow strip assay can be used for on-site detection and rapid preliminary screening of VIR M1 in feed samples.