Abstract
An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional method. The overall hybridization efficiency was increased 3.1-5.2 times. This simple new protocol can reduce the consumption of scarce DNA samples.
Cite
CITATION STYLE
Hirayama, H., Tamaoka, J., & Horikoshi, K. (1996). Improved immobilization of DNA to microwell plates for DNA-DNA hybridization. Nucleic Acids Research, 24(20), 4098–4099. https://doi.org/10.1093/nar/24.20.4098
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