Microtiter assay for interferon: microspectrophotometric quantitation of cytopathic effect

Abstract

This report describes an accurate, reproducible, and efficient microassay for human interferon, using a dye uptake method to quantitate cytopathogenicity. The antiviral activity was measured by using a Gilford 300 N microspectrophotometer, with the automatic programmer, sampler, and data lister. Two hundred interferon samples, each in a final volume of 1.0 ml, may be analyzed and recorded in 45 min. The reproducibility of a laboratory reference interferon and the human international reference B 69/19 on two different cell lines, using the model Q Oxford sampler, was found to be excellent, with the standard error of the log10 geometric mean of both references ± 0.04 to 0.06.