Application of cloud point method for spectrophotometric determination of salbutamol sulphate and methyldopa

Abstract

A simple and efficient cloud point spectrophotometric method has been used for the determination of salbutamol sulphate and methyldopa both in pure and pharmaceutical preparations. The procedure was based on the ion association formation with eosin Y. The extraction of ion association, drown to Triton X-114 micelles, was measured spectrophotometrically. The phase separation was studied and optimized. Beer's law was rectilinear over the concentration ranges of 0.1-20 and 0.3-10 µg/mL with molar absorptivity 4x104 and 5.7x104 L.mol-1 cm-1 and average recovery 98.21% and 101.27% for the above drugs, respectively. The method was applied successfully for the determination of salbutamol sulphate and methyldopa in pharmaceuticals.