Microbiological Contamination Linked to the Usage of Mascara among Waterproof and Non-Waterproof Brands and Tear Film Assessment

Abstract

Introduction and Aim: Microorganisms are normally found on human eyelashes. Applying mascara on lashes has the potential to infect the mascara tube with microorganisms. Constant use of eye cosmetics may also affect tear film stability, resulting in decreased tear production. This pilot study in a real-world context looked at the microbiological contamination of two mascara brands worn daily for two months, as well as tear assessment before and after application. Methods and Materials: Sixty women aged 18 to 39 were randomly allocated to apply one of two brands of waterproof mascara or a non-waterproof brand on both upper and lower eyelids every day for two months. Microbial contamination for various Gram-positive and Gram-negative organisms was detected using the streak technique of culture and Gram staining after two months of mascara use. Tears were assessed before and after the first and second months of mascara usage, followed by a post-study questionnaire. Results: There was no growth of bacteria amongst waterproof mascara users. In non-waterproof users, Staphylococcus aureus growth was found in 33.3 of users, Micrococcus growth among 23.3 and both among 15.0. Tear assessment showed a statistically significant difference between before and after the first month of mascara use and a highly statistically significant difference between before and after the second month of mascara use. Conclusion: Both waterproof and non-waterproof mascara are not safe for constant use.