Biossensor amperométrico para determinação de peróxido de hidrogênio em leite

Abstract

An amperometric biosensor was developed for detection of hydrogen peroxide in milk samples. The biosensor was constructed from the immobilization of peroxidase on printed carbon electrode. Optimization parameters were evaluated in order to obtain better performance of the biosensor. The biosensor showed linearity in the range 5.0 to 40.0 μmol L -1H 2O 2 in phosphate buffer. Milk samples without dilution reported the detection limit for H 2O 2 of 0.42 μmol L -1 and quantification of 1.39 μmol L-1.The biosensor could be a sensitive and inexpensive alternative for the detection of H 2O 2 in adulterated milk samples.