Kinetic mechanism of protein N-terminal methyltransferase 1

Abstract

Background: Protein N-terminal methyltransferases (NTMTs) methylate protein N-terminal α-amines. Results: Inhibition pattern and methylation progress analyses were performed to determine the kinetic mechanism and processivity of NTMT1. Conclusion: NTMT1 utilizes a random sequential Bi Bi mechanism and proceeds in a distributive manner. Significance: This information provides a rational basis for developing specific inhibitors targeting NTMT1.