Development and validation of two spectrophotometric methods for simultaneous determination of diosmine and hesperidin in mixture and their applications

Abstract

Two spectrophotometric methods were used to determine two substances simultaneously in a binary mix which are diosmin (DSM) and hesperidin (HSP). First, we used the derivative spectrophotometry by the zero-crossing measurements based on the elaboration of the linear calibration graphs of first derivative values, which are plotted at 269 nm for HSP and 262.5 nm for DSM. Then, for the second method, we opted for the calculation of the peak absorbance ratio λmax of both drug substances (DSM-HSP) in a binary mixture, and the percentage of drugs was determined. Two different ratios were selected. The two methods presented are simple, selective, and reliable, providing satisfactory accuracy. The recoveries obtained in both cases are good and agreed well with the reported values. The results confirmed that the methods can be considered as a good alternative to other costly techniques, in particular, chromatographic techniques. These methods can be used easily and effectively for simultaneous dosing of two active ingredients mixed in pharmaceutical forms with precision. Furthermore, we can use them for monitoring the synthesis of DSM from HSP. Considering the time saved by these methods presented in the brief period of the analysis, we can  use them for daily quality assurance.