The Gingival Crevicular Fluid Levels of IL-1β, IL-6 and TNF-α in Late Adult Rats

Abstract

Aim: To evaluate the levels of  interleukin1β (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) in the samples of gingival crevicular fluid (GCF) taken from the late adult rats during the orthodontic tooth movement and to  evaluate the responses to orthodontic treatment .  Methodology: In experiment 19 adult (120 days) Spraque-Dawley rats were used. Approximately 15 g force applying open coil spring was applied actively between the upper incisors of the rats. Before and after the activation on the 3rd and 7th and 10th days GCF samples were taken from the vestibular surfaces of appliance fixed teeth using periopaper®. Then the samples were biochemically analyzed. For the statistical analysis of working days of each cytokines repetitive variance analysis technique  was used. Results: The levels of IL-1β, IL-6 and TNF-α were the highest in the 3rd day and started to decrease on the 7th and 10th days. Conclusions: The cytokine levels of orthodontic force applied teeth in late adult rats are compatible with the levels of studies in young rats.    How to cite this article: Acun Kaya F, Gündüz Arslan S , Kaya CA, Arslan H, Hamamcı O. The Gingival Crevicular Fluid Levels of IL-1β, IL-6 and TNF-α in Late Adult Rats. Int Dent Res 2011;1:7-12.