Corrigendum: The human milk proteome and allergy of mother and child: exploring associations with protein abundances and protein network connectivity(Front. Immunol., (2022), 13, (977470), 10.3389/fimmu.2022.977470)

Abstract

In the published article, there was an error. The data processing was carried out without setting the fixed propionamide modification for cysteines. In the sample preparation carried out in our study, proteins were reduced and alkylated before tryptic digestion and analysis. This resulted in the modification of the cysteine residues within the sequence. After the nontargeted analysis, this modification should have been taken into account in the settings of the data processing in order to correctly identify all tryptic peptides. The error was that this modification was not set in the software, which resulted in false negative identification of peptides with cysteine residues. Consequently, the identification of proteins was only based on tryptic peptides with unmodified cysteines in their sequence, and proteins rich in cysteines were therefore also identified less. After discovering this error, we reprocessed the raw data, setting the fixed modification in the data processing software. Although this reprocessing resulted in increased identification of cysteine-rich proteins and, consequently, in differences in numbers, tables, figures, and supplementary material, the discussion and conclusions of the article remain the same. Corrections have been made to Materials and methods, Statistical methods, Missing data. This sentence previously stated: In practice this resulted in a minimum of 66 and a median of 215 valid values. The corrected sentence appears below: In practice this resulted in a minimum of 49 and a median of 209 valid values. Corrections have been made to Materials and methods, Statistical methods, Principal component analysis. This sentence previously stated: For unsupervised data exploration, Principal Component Analysis (PCA) (40) was applied on the 300 × 647 data matrix (samples × proteins), using the FactoMineR package for R (41). The corrected sentence appears below:.