Impact of age and heterophilic interference on the basal serum tryptase, a risk indication for anaphylaxis, in 1,092 dermatology patients

Abstract

A raised baseline serum tryptase is a risk indicator for anaphylactic reactions, especially in patients with hy-menoptera venom allergy. Borderline elevations (> 11.4 μg/l) occur frequently and may necessitate invasive diagnostic procedures to rule out systemic mastocy-tosis. We retrospectively analysed 1,092 non-masto-cytotic patients from our general dermatology clinic with respect to age- and gender-associated effects and investigated the impact of heterophilic antibody interference on the tryptase assay. The results were stra-tifed by gender and fve age classes. Sera with raised tryptase (n = 106) were re-tested after pre-incubation with Heterophilic Blocking Tubes (HBT®, Scantibodies Laboratory; Santee, CA, USA). A signifcant increase in baseline tryptase was observed with increasing age. Incubation with HBT® caused a decline of more than 50% in only one case. In conclusion, older patients showed signifcantly higher serum tryptase levels and heterophilic interference was of subordinate relevance. © 2012 The Authors.