ANTIOXIDANT ACTIVITIES AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT FROM SURIAN LEAVES (TOONA SINENSIS)

Abstract

Objective: The objective of the present study is to determine antioxidant activities and phytochemical screening of ethanol extract from surian leaves (Toona sinensis). Methods: This study evaluated the antioxidant activity and conducted phytochemical screening of ethanol extracts obtained from surian leaves. Phytochemical screening was performed using qualitative Thin-Layer Chromatography (TLC), and antioxidant activity was assessed using the DPPH method. Results: The results revealed the presence of polyphenolic compounds (153.10±0.310 mg/g), tannins, flavonoids (33.19±0.890 mg/g), monoterpenoids, quinones, and saponins with Rf values of 0.607±0.019 (flavonoids) and 0.958±0.019 (terpenoids) 0.513±0.038 (steroids) 0.418±0.019 (phenolics). The antioxidant activity test of the ethanol extract from Surian leaves yielded an IC50 value of 12.351±0.092 ppm, which closely matches the IC50 value of the reference vitamin C (7.805±0.686 ppm). Conclusion: In conclusion, based on research methods, the ethanol extract of surian leaves contains flavonoid and phenolic compounds which show strong antioxidant activity.