The nature of the energy requirement for irreversible adsorption of phages T1 and phi 80 was studied by using various specific energy inhibitors and mutants lacking either the Ca2+, Mg2+ adenosine triphosphatase or the ability to produce cytochromes in the absence of added 5 aminolevulinic acid. It was found that irreversible adsorption could be energized both through the electron transport chain and from adenosine 5' triphosphate via the Ca2+, Mg2+ adenosine triphosphatase, indicating the involvement of the energized membrane state. These results and the discovery that phages T1 and phi 80 adsorb reversibly to the isolated tonA gene product are discussed in terms of the possible involvement of functions expressed by the tonB gene region in irreversible adsorption and the relationship to iron transport.
CITATION STYLE
Hancock, R. E. W., & Braun, V. (1976). Nature of the energy requirement for the irreversible adsorption of bacteriophages T1 and Phi80 to Escherichia coli. Journal of Bacteriology, 125(2), 409–415. https://doi.org/10.1128/jb.125.2.409-415.1976
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