Root and leaf abscisic acid concentration impact on gas exchange in tomato (Lycopersicon esculentum mill) plants subjected to partial root-zone drying

Abstract

Partial root-zone drying (PRD) is a deficit irrigation technique with great potential for water saving. A split-root experiment was conducted on tomato in controlled environment in order to test the response of two long-time storage cultivars to PRD. Ponderosa tomato, a cultivar with yellow fruits, was compared to Giallo tondo di Auletta, a local cultivar from southern Campania (Italy). Plants were subjected to three irrigation treatments: plants receiving an amount of water equivalent to 100% of plant evapotranspiration (V100); plants in which 50% of the amount of water given to V100 was supplied (V50); and plants where one root compartment was irrigated at 50% of water requirements and the other compartment was allowed to dry, and thereafter every side was rewetted alternatively (PRD). The highest levels of leaf abscisic acid (ABA) [on average equal to 104 ng g-1 fresh weight FW)] were measured in PRD and V50, at 70 days after transplantation. Root ABA concentration in both PRD and V50 reached mean values of 149 ng g-1 FW. There were differences for the irrigation regime in root ABA biosynthesis and accumulation under partial root-zone drying and conventional deficit irrigation (V50). Assimilation rate, stomatal conductance and intercellular CO2 concentration decreased in relation to the irrigation regime by 22, 36 and 12%, respectively, in PRD, V50 and V100 at 50 days after transplantation. Ponderosa variety accumulated 20% more dry matter than Auletta and significant differences were observed in leaf area. In both PRD and V50 of the two varieties, it was possible to save on average 46% of water. Our results indicate that there is still space to optimise the PRD strategy, to further improve the cumulative physiological effects of the root-sourced signaling system.