Antioxidant activity of ethanol extract and fractions of Piper crocatum with Rancimat and cuprac methods

Abstract

Objectives: Bioactive compounds of Piper crocatum Ruiz & Pav, which have acted as antioxidants can be used to prevent and treat degenerative diseases such as hyperglycemia, cancer, gout and hypertension. This research aimed to determine the highest antioxidant activity from extract and fractions of P. crocatum leaves and to identify the active compounds such as antioxidants. Methods: The extractionwas performed bymacerationwith 70% ethanol and then the crude extract was fractionated with three solvents, namely n-hexane, ethyl acetate and water. The identification of antioxidant activity was carried out using Rancimat and CUPRAC. The active compounds was identified using LC-MS (Liquid Chromatography-Mass Spectrometry). Results: The highest of the Rancimat method was obtained from the ethyl acetate fraction with a protective factor value of 1.38. Ten compounds were identified in the ethyl acetate fraction of P. crocatum leaves. An antioxidant activity according to the CUPRAC method showed the highest antioxidant activity in the sample of the n-hexane fraction with a value of 31.9 μmol Trolox/g extract. Thirteen compounds were identified in the n-hexane fraction of P. crocatum leaves. Conclusions: The highest antioxidant activity was obtained from ethyl acetate and n-hexane fraction. Various active compounds was identified in the highest value sample.