MicroRNA-145-5p suppresses fascin to inhibit the invasion and migration of cervical carcinoma cells

Abstract

MicroRNA s (miRs) can affect the progression of cervical cancer (CC ). The present study investigated the function of miR- 145-5p in CC and demonstrated its association with fascin (FSCN 1). The expression levels of miR- 145-5p in CC tissues and cell lines were analyzed using reverse transcription-quantitative PCR , and its direct targets were explored using a luciferase reporter assay. The viability, migration and invasion of HeLa cells transfected with small interfering FSCN 1 or with miR- 145-5p mimics and inhibitors were analyzed using Cell Counting Kit-8 and Transwell assays. The expression levels of FSCN 1 mRNA and protein were investigated using reverse transcription PCR and western blotting. miR- 145-5p was downregulated in CC tissues and cell lines. Moreover, overexpression of miR- 145-5p inhibited the migration, invasion and viability of HeLa cells. miR- 145-5p directly targeted FSCN 1, which regulated the suppressive functions of miR- 145-5p in CC cells. Overall, miR- 145-5p is a tumor suppressor gene and a promising target for CC treatment.