The myocyte expression of adiponectin receptors and PPAR is highly coordinated and reflects lipid metabolism of the human donors

Abstract

Muscle lipid oxidation is stimulated by peroxisome proliferator-activated receptor (PPAR) δor adiponectin receptor signalling. We studied human myocyte expression of the PPAR and adiponectin receptor genes and their relationship to lipid parameters of the donors. The mRNA levels of the three adiponectin receptors, AdipoR1, AdipoR2, and T-cadherin, were highly interrelated (r≥0.91). However, they were not associated with GPBAR1, an unrelated membrane receptor. In addition, the adiponectin receptors were positively associated with PPAR expression (r≥0.75). However, they were not associated with PPAR. Using stepwise multiple linear regression analysis, PPAR was a significant determinant of T-cadherin (P=.0002). However, pharmacological PPAR activation did not increase T-cadherin expression. The myocyte expression levels of AdipoR1 and T-cadherin were inversely associated with the donors' fasting plasma triglycerides (P